Situation professionnelle
Expériences professionnelles
System process engineer
MERCK MILLIPORE , Molsheim - CDD
Depuis le 19 octobre 2015
Engineer (intern) - upstream process sciences
UCB , Braine l'alleud
De Février 2015 à Juillet 2015
UCB Pharma, Brussels, Belgium is a biopharmaceutical company dedicated to the research, development and commercialization of innovative medicines with a focus on the fields of central nervous system and immunology disorders.
Activities:
- Animal cell culture in stirred bioreactors (fed-batch): cell thawing, cell expansion in shake flasks and waves, equipment setup, antibody production in 80-L bioreactors.
- Process monitoring and process related documentation: fill up and review of batch records.
- Harvest process optimization: disk stack centrifugation, depth and membrane filtration.
- Pre-clinical batch manufacturing: process monitoring, primary recovery (GMP –like).
- Selection of a secondary supplier for harvest filters at 2,000-L scale: filter assessment at bench-scale, first sizing at 2,000-L scale.
- Product quality analysis: titer measurement (HPLC, Octet), antibody purification (Äkta Xpress), charge variant analysis (iCE3), size exclusion chromatography (Acquity UPLC).
Assistant engineer
QIAGEN MARSEILLE , Marseille cedex 9
De Septembre 2013 à Février 2014
- Quality improvement of a test based on RNA quantification (feasibility stage): RT-qPCR (Rotor Gene Q), selection of new RT and qPCR reagents, new control assessment, reports writing.
- Comparison of three RNA extraction methods (column, trizol)
Laboratory technician (internship)
INSERM , Nice cedex 3 - Stage
De Avril 2012 à Juin 2012
Subject: effect of the Peroxisome Proliferator-Activated Receptor Beta (PPARb) pathway after activation in primary cells (mouse CD4+ T lymphocyte and JURKAT human leukemic T cell line). Effect on metabolism and immunity.
- RNA quantification: RNA isolation, RT-qPCR (96-well plates)
- Protein quantification: Western Blot
Parcours officiels
Langues
Anglais - Courant
Français - Langue maternelle
Allemand - Courant